Fig 2: Serum levels of 25(OH)D3, lithocholic acid, and calcium and the expression levels of CYP2R1, CYP27B1, and CYP24A1 in the liver and kidney of plateau zokor at different altitudes. (A–C) represent the serum levels of 25(OH)D3, lithocholic acid, and calcium, respectively. The mRNA (D) and protein (G) expression levels of CYP2R1 in the liver. The mRNA (E) and protein (H) expression levels of CYP27B1 in the kidney. The mRNA (F) and protein (I) expression levels of CYP24A1 in the kidney. ZH and ZL represent the plateau zokor at elevations of 3700 m and 2700 m, respectively. ** p < 0.01. (original Western blot figures are in Figure S2).

Fig 3: Serum levels of 25(OH)D3, lithocholic acid, and calcium and the expression levels of CYP2R1, CYP27B1, and CYP24A1 in the liver and kidney of plateau zokor, plateau pika, and Sprague-Dawley (SD) rats. (A–C) represent the serum levels of 25(OH)D3, lithocholic acid, and calcium, respectively. The mRNA (D) and protein (G) expression levels of CYP2R1 in the liver. The mRNA (E) and protein (H) expression levels of CYP27B1 in the kidney. The mRNA (F) and protein (I) expression levels of CYP24A1 in the kidney. Z, P, and R represent the plateau zokor at 3700 m elevation, plateau pika at 3700 m elevation, and SD rat at 1500 m elevation, respectively. Significant differences among species are represented by different lowercase letters (p < 0.05). No significant differences among species are represented by the same lowercase letters (p > 0.05). (original Western blot figures are in Figure S1).